Burkholderia pseudomallei Requires Zn(sup2+) for Optimal Exoprotease Production in Chemically Defined Media.

Complete Killing of Caenorhabditis elegans by Burkholderia pseudomallei Is Dependent on Prolonged Direct Association with the Viable Pathogen

BACKGROUND Burkholderia pseudomallei is the causative agent of melioidosis, a disease of significant morbidity and mortality in both human and animals in endemic areas. Much remains to be known about the contributions of genotypic variations within the bacteria and the host, and environmental factors that lead to the manifestation of the clinical symptoms of melioidosis. METHODOLOGY/PRINCIPAL...

Comparison of Mast Burkholderia Cepacia, Ashdown + Gentamicin, and Burkholderia Pseudomallei Selective Agar for the Selective Growth of Burkholderia Spp.

Reliable identification of pathogenic Burkholderia spp. like Burkholderia mallei and Burkholderia pseudomallei in clinical samples is desirable. Three different selective media were assessed for reliability and selectivity with various Burkholderia spp. and nontarget organisms. Mast Burkholderia cepacia agar, Ashdown + gentamicin agar, and B. pseudomallei selective agar were compared. A panel o...

Exoprotease Production by Aeromonas Hydrophila in a Chemically Defined Medium Thesis

Design and production of a genetically simulant construct for the detection of B. malei and pseudomaleii

Aim & Background the genus Burkholderia (mallei and pseudomallei species) are classified as dangerous human pathogens. Diagnosing cultures for this bacterium is costly and time consuming and on the other hand it is very dangerous to work with and it is very difficult to access this bacterium. the porpose of this research Design and production of a genetically simulant construct for the detectio...

The BpsIR quorum-sensing system of Burkholderia pseudomallei.

BpsIR, a LuxIR quorum-sensing homolog, is required for optimal expression of virulence and secretion of exoproducts in Burkholderia pseudomallei. Cell density-dependent expression of bpsI and bpsR, the positive regulation of bpsIR expression by BpsR, and the synthesis of N-octanoyl-homoserine lactone (C8HSL) by BpsI are described in this report.